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Oligonucleotide synthesis is the process of chemically creating short DNA or RNA sequences in the laboratory. It involves the step-by-step assembly of nucleotides to form the desired sequence. Once the synthesis by the oligo synthesizer machine is complete, purification methods are employed to isolate and obtain pure oligonucleotides for various applications, such as research, diagnostics, and therapeutic development.
The purity of synthesized oligonucleotides is typically assessed using techniques such as high-performance liquid chromatography (HPLC) or capillary electrophoresis (CE). These methods can separate and quantify impurities such as incomplete sequences, truncated products, and deletions. Analytical techniques like mass spectrometry can also be used to confirm the identity and molecular weight of the synthesized oligonucleotides. Learn more about our oligonucleotide synthesis machine and get price.