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The purified antibodies can then undergo additional processing steps, including concentration, buffer exchange, and sterile filtration, to obtain the final product ready for formulation, characterization, and subsequent use in various applications.
The purity of purified antibodies is typically assessed using analytical techniques such as SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), capillary electrophoresis, size exclusion chromatography, or high-performance liquid chromatography (HPLC). These methods help confirm the absence of impurities and determine the purity and integrity of the antibodies.